Glucose oxidase oxidizes glucose to gluconic acid and

Glucose oxidase oxidizes glucose to gluconic acid and

• A. H2O2
• B. CO2
• C. HCO3
• D. H2O

Answer: (A)

Glucose oxidase takes electrons from glucose and donates them to molecular oxygen, producing gluconic acid and hydrogen peroxide as the byproduct. The enzyme uses a flavin cofactor (FAD), which gets reduced as glucose is oxidized and then reoxidized by O2, releasing H2O2. This hydrogen peroxide is a hallmark of the reaction and is also why GOx is useful in glucose sensing, often paired with peroxidase for detection. The other substances listed don’t arise from this specific enzymatic step: CO2 would come from complete oxidation in respiration, bicarbonate comes from CO2 in solution, and water would be formed in different redox contexts, not this GOx-catalyzed pair.

Glucose oxidase takes electrons from glucose and donates them to molecular oxygen, producing gluconic acid and hydrogen peroxide as the byproduct. The enzyme uses a flavin cofactor (FAD), which gets reduced as glucose is oxidized and then reoxidized by O2, releasing H2O2. This hydrogen peroxide is a hallmark of the reaction and is also why GOx is useful in glucose sensing, often paired with peroxidase for detection. The other substances listed don’t arise from this specific enzymatic step: CO2 would come from complete oxidation in respiration, bicarbonate comes from CO2 in solution, and water would be formed in different redox contexts, not this GOx-catalyzed pair.