Substances known to increase results when measuring creatinine by the Jaffe reaction include all of the following EXCEPT

Substances known to increase results when measuring creatinine by the Jaffe reaction include all of the following EXCEPT

• A. Ascorbic acid
• B. Bilirubin
• C. Glucose
• D. α-Keto acids

Answer: (A)

The Jaffe reaction measures creatinine by its reaction with picric acid in an alkaline medium to form a colored complex; because many other substances can affect this color development or add their own color, the method is prone to interference that can falsely raise measured creatinine. Substances like bilirubin, glucose, and α-keto acids can contribute to the color or react with the reagents in a way that boosts the signal, causing higher results than true creatinine. Ascorbic acid, on the other hand, tends to reduce the color formed by the Jaffe reaction rather than strengthen it, so it does not cause the falsely elevated results that the others can. Therefore, ascorbic acid is the one that does not increase the measured creatinine by this method.

The Jaffe reaction measures creatinine by its reaction with picric acid in an alkaline medium to form a colored complex; because many other substances can affect this color development or add their own color, the method is prone to interference that can falsely raise measured creatinine. Substances like bilirubin, glucose, and α-keto acids can contribute to the color or react with the reagents in a way that boosts the signal, causing higher results than true creatinine. Ascorbic acid, on the other hand, tends to reduce the color formed by the Jaffe reaction rather than strengthen it, so it does not cause the falsely elevated results that the others can. Therefore, ascorbic acid is the one that does not increase the measured creatinine by this method.